What Challenges Exist in Studying DNA Polymerase Catalysis?
Studying DNA polymerase catalysis presents challenges such as: - Structural Complexity: The intricate structure and dynamic conformational changes can be difficult to capture. - Kinetic Analysis: Measuring the rapid catalytic cycles and intermediate states requires sophisticated techniques. - Mutagenesis Effects: Understanding how mutations affect catalysis and fidelity requires comprehensive biochemical and structural studies.
