1. Resolution: MeDIP provides relatively low resolution compared to other techniques like whole-genome bisulfite sequencing. It generally identifies regions of methylation rather than single-base resolution. 2. Antibody Specificity: The effectiveness of MeDIP depends on the specificity and affinity of the antibody used. Non-specific binding can lead to false positives. 3. Quantitative Limitations: MeDIP is not inherently quantitative, so additional techniques such as qPCR or sequencing are required to quantify methylation levels.
