1. DNA Extraction: Genomic DNA is extracted from cells or tissues of interest. 2. Shearing: The DNA is sheared into smaller fragments, typically 300-1000 bp, using sonication or enzymatic digestion. 3. Immunoprecipitation: The fragmented DNA is incubated with an antibody specific to 5-methylcytosine (5mC), allowing the antibody to bind to methylated DNA. 4. Pull-Down: The antibody-DNA complexes are captured using Protein A/G beads. 5. Washing and Elution: Unbound DNA is washed away, and the methylated DNA is eluted from the beads for subsequent analysis.
