The bisulfite sequencing process typically involves several key steps:
DNA Isolation: The first step involves isolating DNA from the sample of interest. Bisulfite Treatment: The isolated DNA is treated with sodium bisulfite, converting unmethylated cytosines to uracil. PCR Amplification: The bisulfite-treated DNA is then amplified using PCR, which converts uracil to thymine. Sequencing: The amplified DNA is sequenced using high-throughput sequencing technologies. Data Analysis: The sequencing data is analyzed to determine the methylation status of cytosines.
