Advancements in catalysis can address some of the challenges in bisulfite sequencing:
Enhanced Catalysts: Developing more efficient chemical catalysts can improve the specificity and efficiency of the bisulfite treatment, reducing DNA degradation and incomplete conversion. Enzymatic Catalysis: Enzymes such as bisulfite-specific deaminases can be engineered to facilitate the selective conversion of cytosine to uracil, minimizing sequence biases and improving overall accuracy. Optimized Reaction Conditions: Fine-tuning the reaction conditions, such as pH and temperature, can enhance the catalytic efficiency of the bisulfite treatment process.
