bisulfite sequencing

What are the Challenges in Bisulfite Sequencing?

Despite its powerful applications, bisulfite sequencing faces several challenges:
DNA Degradation: The bisulfite treatment process can lead to DNA degradation, which can complicate subsequent analysis.
Incomplete Conversion: Ensuring complete conversion of unmethylated cytosines to uracil is critical for accurate methylation analysis.
Sequence Bias: The bisulfite treatment can introduce sequence biases, affecting the accuracy of the methylation data.

Frequently asked queries:

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