How is Catalysis Involved in Bisulfite Sequencing?
Catalysis plays a critical role in the bisulfite sequencing process, particularly during the conversion of cytosine to uracil. Enzymes and chemical catalysts facilitate the reaction conditions required for the bisulfite treatment to be effective. These catalysts ensure that the bisulfite reagent selectively deaminates cytosine residues without causing excessive DNA degradation or nonspecific reactions.
