What Analytical Techniques are Used in In Vitro Assays for Catalysis?
Several analytical techniques are employed to monitor and analyze catalytic reactions:
1. Gas Chromatography (GC): Used for separating and analyzing compounds that can be vaporized. 2. High-Performance Liquid Chromatography (HPLC): Ideal for separating and quantifying non-volatile compounds. 3. Mass Spectrometry (MS): Provides detailed information about the molecular weight and structure of the compounds. 4. Nuclear Magnetic Resonance (NMR) Spectroscopy: Useful for elucidating the structure of organic compounds involved in the reaction. 5. UV-Visible Spectroscopy: Monitors the absorbance of light by reactants and products, useful for reactions involving color changes.
