1. Controlled Environment: These assays allow researchers to manipulate and control experimental variables precisely, leading to more reliable and reproducible results. 2. Mechanistic Insights: They help in understanding the mechanistic pathways of catalytic reactions, which is essential for designing better catalysts. 3. Optimization: In vitro assays facilitate the rapid screening and optimization of catalyst formulations and reaction conditions. 4. Cost-Effective: Conducting experiments in vitro is often less expensive and less time-consuming than in vivo studies.