In vitro assays in catalysis typically involve the following steps:
1. Preparation of Catalysts: Catalysts can be prepared in various forms, including nanoparticles, enzymes, or homogeneous catalysts, depending on the type of reaction being studied. 2. Reaction Setup: The catalytic reaction is set up in a reaction vessel, and all necessary reactants and solvents are added. 3. Monitoring: The progress of the reaction is monitored using analytical techniques such as Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), or Spectroscopy. 4. Data Analysis: The data obtained from these techniques are analyzed to determine the catalytic activity, turnover frequency (TOF), and selectivity of the catalyst.
