How do Restriction Enzymes Compare to CRISPR-Cas9?
While restriction enzymes and CRISPR-Cas9 both facilitate targeted DNA cleavage, they differ significantly in their mechanisms and applications. Restriction enzymes recognize specific short sequences and cut within or near these sites. In contrast, CRISPR-Cas9 is a more flexible system that uses an RNA guide to target almost any sequence, allowing for more extensive genome editing capabilities.
