What is the Mechanism of Catalysis in Restriction Enzymes?
The mechanism of catalysis involves several steps:
1. Recognition: The restriction enzyme identifies its specific DNA sequence. 2. Binding: The enzyme binds to the DNA, forming an enzyme-DNA complex. 3. Cleavage: The enzyme catalyzes the hydrolysis of the phosphodiester bonds within the recognition site.
The active site of the enzyme contains residues that stabilize the transition state and facilitate bond cleavage. This often involves metal ions, like magnesium, which are crucial for the catalytic activity.
