How are Michaelis-Menten Parameters Determined Experimentally?
To determine \( V_{max} \) and \( K_m \), one typically performs enzyme assays with varying substrate concentrations and measures the reaction rates. A common approach is to plot the data using a Lineweaver-Burk plot or a Hanes-Woolf plot to linearize the Michaelis-Menten equation, making it easier to extract kinetic parameters.
