What is the Significance of \( V_{max} \) and \( K_m \)?
The parameters \( V_{max} \) and \( K_m \) are essential for characterizing enzyme behavior: - \( V_{max} \) indicates the maximum catalytic activity of the enzyme when the substrate concentration is saturating. - \( K_m \) provides insight into the enzyme's affinity for its substrate. A lower \( K_m \) value indicates higher affinity, meaning the enzyme can achieve half-maximum catalysis at a lower substrate concentration.
