What are the Limitations of Michaelis-Menten Kinetics?
While powerful, the Michaelis-Menten model has limitations: - Simplistic Assumptions: It assumes a single substrate binding and ignores the complexity of multi-substrate reactions. - Steady-State Assumption: It relies on the steady-state assumption, which may not hold true for all enzyme-catalyzed reactions. - Inapplicability to All Enzymes: Not all enzymes follow Michaelis-Menten kinetics, especially those that exhibit cooperative binding or allosteric regulation.
