The use of cell-free systems offers several advantages in the field of catalysis. Firstly, they provide a simplified environment to study catalytic mechanisms without the interference of other cellular processes. This can lead to a deeper understanding of how enzymes work. Secondly, cell-free systems can be easily modified to optimize reaction conditions, such as pH, temperature, and substrate concentration, allowing for greater control over the reaction kinetics. Additionally, these systems facilitate the incorporation of non-natural substrates and cofactors, expanding the range of possible catalytic reactions.
