What is Total Internal Reflection Fluorescence (TIRF)?
Total Internal Reflection Fluorescence (TIRF) is an advanced optical technique used to observe and measure events occurring at or near the interface between two media, typically a glass substrate and an aqueous solution. This method exploits the phenomenon of total internal reflection to generate an evanescent wave that selectively excites fluorescent molecules within a very thin region (typically around 100-200 nm) from the interface. This allows for high-resolution imaging and quantitative analysis of processes occurring at surfaces.
