What Information Can Be Obtained from Lineweaver-Burk Plots?
The primary utility of the Lineweaver-Burk plot is to determine the Michaelis constant (Km) and the maximum reaction velocity (Vmax). The y-intercept of the plot equals 1/Vmax, and the x-intercept equals -1/Km. The slope of the line is Km/Vmax. These parameters are essential for understanding the catalytic efficiency of an enzyme and its affinity for substrates.
