What Are the Limitations of Lineweaver-Burk Plots?
While Lineweaver-Burk plots are useful, they come with certain limitations. One major drawback is the distortion of data due to the reciprocal transformation, which can disproportionately emphasize errors in data points where substrate concentrations are low. This can result in unreliable estimates of Km and Vmax. Additionally, Lineweaver-Burk plots can complicate the analysis of enzymes that exhibit complex kinetic behaviors, such as allosteric enzymes.
