Despite its many advantages, there are some challenges associated with HRP:
Inactivation: HRP can be inactivated by high concentrations of hydrogen peroxide, limiting its use in some applications. Substrate limitation: While versatile, HRP may not be effective for all substrate types, necessitating the search for alternative peroxidases or engineered enzymes. Product inhibition: Accumulation of reaction products can inhibit HRP activity, requiring careful optimization of reaction conditions.
