What are Some Challenges in Studying IDO Catalysis?
Studying IDO catalysis presents several challenges, including the complexity of its reaction mechanism and the transient nature of reaction intermediates. The enzyme's activity can be influenced by various factors, such as substrate concentration, pH, and the presence of cofactors. Additionally, understanding the structural dynamics of the enzyme during catalysis requires advanced techniques such as X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy.
