Understanding how denaturants affect enzymes is crucial for several reasons: 1. Enzyme Stability: In industrial applications, enzymes need to remain stable under various conditions. Knowledge of denaturants helps in engineering more robust enzymes. 2. Drug Design: Many pharmaceuticals target the active sites of enzymes. Denaturants can serve as models to study these interactions. 3. Protein Folding: Insights into denaturation can aid in understanding protein folding mechanisms, which is vital for designing novel biocatalysts.