Several factors contribute to the overall cost of enzyme production. These include:
1. Raw Materials: The substrates and nutrients required for microbial fermentation or other production methods. 2. Production Methods: The choice between microbial fermentation, plant-based production, or synthetic biology. 3. Downstream Processing: The steps involved in purifying and formulating the enzyme. 4. Scale of Production: The economies of scale achieved in large-scale operations. 5. Regulatory Compliance: Meeting the necessary regulatory standards for enzyme production and use.