Several factors impact the activity of enzymes, including: - Temperature: Each enzyme has an optimal temperature range. Deviations can lead to denaturation or reduced activity. - pH: Enzymes also have an optimal pH range where they function most efficiently. - Substrate Concentration: The rate of reaction increases with substrate concentration until it reaches a saturation point. - Inhibitors: Substances that reduce enzyme activity. They can be competitive, non-competitive, or uncompetitive.