What are the Limitations of the Michaelis-Menten Equation?
While the Michaelis-Menten equation is a powerful tool, it has limitations: 1. It does not account for enzyme inhibition or activation, which can significantly impact reaction rates. 2. It is less applicable to multi-substrate reactions or complex enzymatic mechanisms. 3. It assumes a simple one-substrate, one-product reaction, which is often not the case in biological systems.
