Despite its many advantages, HSQC has some limitations: 1. Complexity: The interpretation of HSQC spectra can be complex, requiring expertise in NMR spectroscopy. 2. Sample Requirements: HSQC typically requires relatively high sample concentrations and isotopically labeled samples, which can be expensive and time-consuming to prepare. 3. Time-Consuming: HSQC experiments can be time-consuming, especially when high resolution and sensitivity are required.
