What are the Applications of RNase H in Biotechnology?
RNase H has several applications in biotechnology and molecular biology. It is often used in techniques such as cDNA synthesis, where it removes the RNA strand from RNA-DNA hybrids formed during reverse transcription. This step is crucial for generating stable DNA copies of RNA sequences. Additionally, RNase H is used in antisense technology, where it facilitates the degradation of target RNA molecules hybridized to antisense oligonucleotides, thus regulating gene expression.
