Selecting an appropriate internal standard is critical and involves considering several factors:
1. Chemical Similarity: The internal standard should be chemically similar to the analyte to ensure comparable behavior during the reaction and detection process. 2. Non-Interference: The standard should not interfere with the reaction or the detection of the target analyte. 3. Stability: It should remain stable under the reaction conditions and not decompose or react with the catalyst or other components. 4. Availability: The internal standard should be readily available and relatively inexpensive.
