Where: - \( V \) is the reaction rate. - \( V_{max} \) is the maximum reaction rate. - \([S]\) is the substrate concentration. - \( K_m \) is the Michaelis Constant.
By plotting the reaction rate \( V \) against \([S]\), the Km can be estimated from the substrate concentration at which \( V = \frac{V_{max}}{2} \).
