Despite its potential, enzyme engineering faces several challenges:
1. Stability: Many natural enzymes are not stable under industrial conditions, such as high temperatures and extreme pH levels. Engineering enzymes to withstand these conditions is a significant challenge. 2. Scalability: Producing engineered enzymes at an industrial scale can be complex and costly. 3. Specificity: Achieving the desired level of specificity without sacrificing catalytic efficiency can be difficult. 4. Regulatory Hurdles: Ensuring that engineered enzymes are safe for use in food and pharmaceuticals requires extensive testing and regulatory approval.
