What are the Key Parameters Measured in Kinetic Assays?
Several key parameters are measured in kinetic assays to evaluate catalytic performance: - Reaction Rate (v): The speed at which reactants are converted to products. - Rate Constant (k): A proportionality constant that relates the reaction rate to the concentrations of reactants. - Turnover Number (TON): The number of substrate molecules converted per catalyst molecule. - Turnover Frequency (TOF): The number of turnover events per unit time. - Michaelis-Menten Constants (Km and Vmax): Used in enzyme kinetics to describe the affinity of the enzyme for its substrate and the maximum rate of the reaction.
