Several methods are employed to achieve enzyme overexpression: - Gene Cloning: Inserting multiple copies of the enzyme's gene into the host organism's genome. - Promoter Engineering: Utilizing strong promoters to increase the transcription of the enzyme gene. - Plasmid Vectors: Introducing plasmids carrying the enzyme gene into the host cells. - Codon Optimization: Modifying the gene sequence to match the host organism's preferred codon usage, ensuring efficient translation.
