What are the Challenges Associated with Enzyme Overexpression?
Despite its advantages, enzyme overexpression poses several challenges: - Metabolic Burden: Overexpression can impose a metabolic burden on the host cell, affecting its growth and viability. - Enzyme Stability: High levels of enzyme production may lead to the formation of [insoluble aggregates](https://) or degradation of the enzyme. - Regulatory Constraints: Ensuring controlled and precise overexpression can be difficult, leading to variability in enzyme activity. - Resource Allocation: The cell's resources may be diverted towards enzyme production, affecting other cellular functions.
