The process of homology modeling involves several key steps:
1. Template Identification: The first step is to identify a suitable template protein with a known structure that shares a significant sequence similarity with the target protein. 2. Sequence Alignment: Align the amino acid sequence of the target protein with that of the template protein to ensure the correct mapping of residues. 3. Model Building: Construct the model of the target protein based on the aligned sequences and the template's structure. 4. Model Refinement: Refine the model to correct any structural inconsistencies and to optimize the protein's geometry. 5. Model Validation: Validate the model using various criteria such as Ramachandran plots, to ensure its reliability.
