Lysosomal enzymes are synthesized in the rough endoplasmic reticulum and transported to the lysosome via the Golgi apparatus. These enzymes are inactive at the neutral pH of the cytoplasm but become active in the acidic environment of the lysosome. This ensures that the enzymes do not digest cellular components outside the lysosome. The hydrolases in the lysosome facilitate the breakdown of substrates by adding water molecules to the bonds, thus catalyzing their hydrolysis.
