How Do Enzymatic Catalysts Facilitate DNA Extraction?
Enzymatic catalysts work by specifically targeting and breaking down cellular components. For instance, Proteinase K digests proteins, including those that form the nuclear envelope, thus facilitating the release of DNA. Lysozyme, on the other hand, breaks down the peptidoglycan layer of bacterial cell walls, aiding in the extraction of bacterial DNA. These enzymes operate under mild conditions, which helps preserve the integrity of the DNA.
