Several techniques are employed in mechanistic studies:
Spectroscopic Methods: Techniques such as NMR, IR, and UV-Vis spectroscopy are used to identify intermediates and transition states. Kinetic Studies: These involve measuring the rate of reaction under various conditions to infer details about the reaction mechanism. Isotope Labeling: Using isotopically labeled reactants can help trace the path of atoms through the reaction mechanism. Computational Methods: Quantum mechanical calculations and molecular dynamics simulations can provide detailed insights into the potential energy surface and transition states.
