Fructose 1,6 Bisphosphate Aldolase - Catalysis

What is Fructose 1,6 Bisphosphate Aldolase?

Fructose 1,6 bisphosphate aldolase (commonly referred to as aldolase) is an enzyme that plays a critical role in the glycolytic pathway, which is essential for cellular respiration. It catalyzes the reversible cleavage of fructose 1,6-bisphosphate into two three-carbon products: glyceraldehyde 3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP).

How Does Aldolase Facilitate Catalysis?

Aldolase facilitates catalysis via a mechanism that involves the formation of a Schiff base intermediate between the enzyme and the substrate. The active site of aldolase contains an essential lysine residue that forms a covalent bond with the substrate, stabilizing the transition state and thereby lowering the activation energy required for the reaction to proceed.

What are the Types of Aldolase?

There are three primary isoforms of aldolase: Aldolase A, Aldolase B, and Aldolase C. Aldolase A is predominantly found in muscle tissue, aldolase B is located in the liver, and aldolase C is primarily present in the brain. Each isoform has specific roles adapted to the metabolic requirements of the tissues in which they are expressed.

What is the Biological Significance of Aldolase?

Aldolase is crucial for energy production and metabolic regulation. In the glycolytic pathway, it allows cells to efficiently convert glucose into pyruvate, generating ATP and NADH in the process. This makes aldolase indispensable for both aerobic and anaerobic respiration.

What are the Clinical Implications of Aldolase Deficiency?

A deficiency in aldolase, particularly aldolase B, can lead to serious metabolic disorders such as Hereditary Fructose Intolerance (HFI). Individuals with HFI lack the ability to properly metabolize fructose, leading to severe symptoms such as hypoglycemia, jaundice, and potential liver failure upon ingestion of fructose-containing foods.

How is Aldolase Activity Assessed?

Aldolase activity can be assessed using biochemical assays that measure the rate of conversion of fructose 1,6-bisphosphate to G3P and DHAP. These assays often involve spectrophotometric methods to detect changes in absorbance correlated with the formation of reaction products.

What are the Applications of Aldolase in Biotechnology?

In biotechnology, aldolase enzymes have been exploited for various applications including biosensors, biofuel production, and synthetic biology. Their ability to catalyze specific biochemical reactions makes them valuable tools for engineering metabolic pathways in microorganisms for the production of high-value compounds.

Relevant Publications

Age-Related Changes in the Glycolytic Enzymes of M2-Isoform of Pyruvate Kinase and Fructose-1,6-Bisphosphate Aldolase: Implications to Age-Related Macular Degeneration.

Issue Release: 2024

Overexpression of the and genes promotes high production of HDMF in .

Issue Release: 2024

A novel gluconeogenic route enables efficient use of erythritol in zoonotic .

Issue Release: 2024

Unveiling the Biological Function of FBA6 () through the Identification of the Fructose-1,6-Bisphosphate Aldolase Gene.

Issue Release: 2024

PoWRKY69-PoVQ11 module positively regulates drought tolerance by accumulating fructose in Paeonia ostii.

Issue Release: 2024

Peptostreptococcus stomatis promotes colonic tumorigenesis and receptor tyrosine kinase inhibitor resistance by activating ERBB2-MAPK.

Issue Release: 2024

Proteomic Profiling of Dilated Cardiomyopathy Plasma Samples ─ Searching for Biomarkers with Potential to Predict the Outcome of Therapy.

Issue Release: 2024

Identification and characterization of FBA genes in moso bamboo reveals PeFBA8 related to photosynthetic carbon metabolism.

Issue Release: 2024

Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation.

Issue Release: 2024

Discovery of petroselinic acid with in vitro and in vivo antifungal activity by targeting fructose-1,6-bisphosphate aldolase.

Issue Release: 2024

A Nanobody/Monoclonal Antibody \"hybrid\" sandwich technology offers an improved immunoassay strategy for detection of African trypanosome infections.

Issue Release: 2024

Photosynthesis regulates tillering bud elongation and nitrogen-use efficiency via sugar-induced NGR5 in rice.

Issue Release: 2024

Polybrominated diphenyl ethers and polychlorinated biphenyls induced rice \"diabetes\" by disturbing the transport and decomposition of soluble sugars.

Issue Release: 2024

Genome-Wide Characterization of Fructose 1,6-Bisphosphate Aldolase Genes and Expression Profile Reveals Their Regulatory Role in Abiotic Stress in Cucumber.

Issue Release: 2024

Nitric oxide mediates positive regulation of Nostoc flagelliforme polysaccharide yield via potential S-nitrosylation of G6PDH and UGDH.

Issue Release: 2024

The fructose-bisphosphate, Aldolase A (ALDOA), facilitates DNA-PKcs and ATM kinase activity to regulate DNA double-strand break repair.

Issue Release: 2023

Elevated inorganic carbon and salinity enhances photosynthesis and ATP synthesis in picoalga as revealed by label free quantitative proteomics.

Issue Release: 2023

Thermal Ring-Based Heat Switches in Hyperthermophilic Class II Bacterial Fructose Aldolase.

Issue Release: 2023

Discovery of a novel transcriptional regulator of sugar catabolism in archaea.

Issue Release: 2023

A Manganese-independent Aldolase Enables Staphylococcus aureus To Resist Host-imposed Metal Starvation.

Issue Release: 2023

What Tools and Platforms Facilitate Collaboration?

What are Some Recent Innovations in Catalysis?

How Do Catalysts Affect the Environment?

What are Single Molecule Studies?

Why is Conductivity Important in Catalysis?

What Challenges Are Faced in Identifying Active Sites?

What Factors Affect Reliability?

What Are the Challenges of Screen Sharing in Catalysis?

What Role Do Functional Groups Play?

What Factors Influence Δ?

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