In the study of catalytic reactions, accurate determination of kinetic parameters is crucial. Traditional methods like the Michaelis-Menten plot often lead to errors due to the uneven distribution of data points. The Eadie-Hofstee plot, which plots the reaction velocity (v) against v/[S] (where [S] is the substrate concentration), provides a more uniform spread of data points and reduces the statistical weight of outliers, thereby giving a more reliable estimation of Vmax and Km.
