What Techniques are Used to Mitigate Fluorescence Interference?
Several strategies can be employed to minimize fluorescence interference in catalytic studies: - Spectroscopic Methods: Techniques such as time-resolved fluorescence spectroscopy or fluorescence quenching can help distinguish between the signal of interest and background fluorescence. - Sample Preparation: Purifying reagents and selecting non-fluorescent solvents can reduce interference. - Instrumentation: Advanced instruments with high sensitivity and selectivity can help in distinguishing the catalyst's signal from background fluorescence. - Mathematical Corrections: Computational methods and data processing techniques can be used to subtract background fluorescence from the data.
