How Does Fluorescence Interference Affect Reaction Monitoring?
Fluorescence interference can significantly impact the monitoring and analysis of catalytic reactions. It can lead to: - Misinterpretation of Kinetics: Overlapping signals can obscure the true reaction rates. - Erroneous Mechanistic Insights: Misidentification of intermediates can lead to incorrect mechanistic pathways. - Reduced Sensitivity: High background noise can decrease the sensitivity of detection, making it challenging to observe low-concentration species.
