Nitrile hydratase (NHase) is an enzyme that catalyzes the hydration of nitriles to their corresponding amides. This biocatalyst is particularly valuable in industrial applications due to its ability to transform nitriles under mild conditions, making it a sustainable alternative to traditional chemical processes. NHase is composed of two subunits, typically referred to as α and β, and contains either a cobalt or iron ion at its active site.