NHases are classified based on the metal ion present in their active site. The two main types are:
- Cobalt-containing NHase: This type is prevalent in many bacterial species and is highly active and stable. - Iron-containing NHase: This type is also found in various bacterial species and exhibits similar catalytic properties to its cobalt counterpart.
Both types have been extensively studied, and their structures have been elucidated through X-ray crystallography, revealing insights into their catalytic mechanisms and substrate specificities.
