One of the primary challenges in utilizing Frh is its sensitivity to oxygen, which can inactivate the enzyme. This limitation necessitates the development of oxygen-tolerant variants or the implementation of anaerobic conditions in industrial processes. Another challenge is the large-scale production of the enzyme, which requires efficient expression systems and purification techniques. Despite these challenges, ongoing research aims to overcome these obstacles and harness the full potential of Frh in biotechnological applications.
