What Challenges Are Associated with Electron Tomography?
Despite its advantages, electron tomography also has some challenges. One of the main difficulties is the beam damage to the sample, which can occur due to the high-energy electron beam used in TEM. This is especially problematic for sensitive materials that may degrade under prolonged exposure. Additionally, the reconstruction process can be computationally intensive and may require sophisticated algorithms to accurately model the 3D structure. Another challenge is the limited tilt range, known as the "missing wedge," which can lead to artifacts in the reconstructed 3D image.
