What Are the Limitations of UV-Vis Spectrophotometry?
While UV-Vis Spectrophotometry is a versatile and powerful tool, it has certain limitations:
Sample Transparency: The sample must be transparent in the UV-Vis range, which can be a limitation for some solid catalysts. Concentration Range: The technique is most effective within a certain concentration range, and very high or very low concentrations can lead to inaccuracies. Overlapping Absorption Peaks: In complex mixtures, overlapping absorption peaks can make it challenging to deconvolute the spectra and identify individual species.
