Several key parameters must be evaluated during method validation:
Accuracy: The closeness of the measured values to the true value. Precision: The degree of agreement among repeated measurements. Selectivity: The ability to distinguish the analyte from other components in the matrix. Sensitivity: The capability of detecting small quantities of the analyte. Linearity: The method's ability to elicit test results that are directly proportional to the concentration of analyte in samples. Robustness: The capacity to remain unaffected by small, deliberate variations in method parameters. Reproducibility: The method's consistency when performed by different operators, using different equipment, or in different laboratories.
