What are the Different Types of Fluorescence Imaging Technologies?
There are several fluorescence imaging technologies utilized in the study of catalysis:
Confocal Microscopy: Provides high-resolution images by focusing light to a single point and collecting emitted fluorescent light through a pinhole. Total Internal Reflection Fluorescence (TIRF) Microscopy: Ideal for studying surface-bound processes by exciting fluorophores only near the surface. Fluorescence Lifetime Imaging Microscopy (FLIM): Measures the lifetime of the excited state of fluorophores, offering insights into the local environment and interactions. Super-Resolution Microscopy: Techniques like STED, PALM, and STORM break the diffraction limit, providing nanometer-scale resolution.