There are several types of ELISAs, each serving different applications and offering varying levels of sensitivity and specificity:
Direct ELISA: Involves the direct attachment of the enzyme-conjugated antibody to the target antigen. Indirect ELISA: Uses a secondary antibody conjugated to an enzyme to detect the primary antibody bound to the antigen. Sandwich ELISA: Employs two antibodies, a capture antibody and a detection antibody, to sandwich the target antigen. Competitive ELISA: Involves the competition between the target antigen and a labeled antigen for binding to an antibody.
