What Are the Challenges in Using Fusion Proteins for Catalysis?
While fusion proteins offer numerous advantages, they also present several challenges:
Structural Stability: The fusion of different protein domains can lead to issues with folding and stability, potentially reducing catalytic efficiency. Expression Levels: Achieving high expression levels of functional fusion proteins can be difficult, particularly in heterologous systems. Optimization: Designing fusion proteins requires careful optimization to ensure that the individual domains retain their functional properties without interfering with each other.
